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Cat. No.
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CPE08
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Description
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Peroxidase (HRP) is a hemoprotein catalyzing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome C and the leuco form of many dyes.
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CAS No.
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9003-99-0
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Synonym
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myeloperoxidase; lactoperoxidase; verdoperoxidase; guaiacol peroxidase; thiocyanate peroxidase; eosinophil peroxidase; Japanese radish peroxidase; horseradish peroxidase (HRP); extensin peroxidase; heme peroxidase; MPO; oxyperoxidase; protoheme peroxidase; pyrocatechol peroxidase; scopoletin peroxidase; donor:hydrogen-peroxide oxidoreductase; peroxidase.
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Reaction
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donor + H2O2 = oxidized donor + 2 H2O
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Molecular Weight
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40,000Da
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Unit Definition
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One Worthington Unit decomposes 1µmole of H2O2 per minute at 25°C, pH 7.0 using aminoantipyrine and phenol.
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Composition
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Seven isozymes have been described. All contain photohemin IX as prosthetic group. Neutral and amino sugars account for approximately 18% of the enzyme. The "active site" involves apoprotein as well as the heme group.
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Optimum pH
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7.0
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Isoelectric point
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7.2
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Stability
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The enzyme is quite stable. As a lyophilized powder, it may be stored several years refrigerated.
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Specificity
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The enzyme exhibits a high specificity. Activity is observed with H2O2, MeOOH, and EtOOH.
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Inhibitors
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Horseradish peroxidase is reversibly inhibited by cyanide and sulfide at a concentration of 10-5 M
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Applications
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ELISA, Western Blot and Immunoblotting
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Storage
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Store in an airtight container, at a temperature of 2°C to 8°C.
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Product Specification(Ⅰ)
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Appearance
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Brown lyophilized powder
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Activity(Sigma-aldrich)
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≥ 300 IU/mg
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RZ(Sigma-aldrich)
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≥ 3.0
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Specification(Ⅱ)
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Appearance
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Brown lyophilized powder
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Activity(Sigma-aldrich)
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≥ 250 IU/mg
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RZ(Sigma-aldrich)
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≥2.5
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Specification(Ⅲ)
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Appearance
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Brown lyophilized powder
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Activity(Sigma-aldrich)
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≥ 150 IU/mg
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RZ(Sigma-aldrich)
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≥ 1.5
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Download Datasheet:
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